The last trick of p53: early 3D chromatin remodelling to trigger cellular stress response

The simplest explanation of cancer is that, for any reason, a cell in our body ignores its biological program and gets loose, multiplying itself again and again, creating a tumour. This has a lot to do with a wrong use of its genetic information, contained within its genes. Cells are smart enough to see this coming and try to activate an emergency response, usually through specific genes called tumour suppressors. The best-known of these is TP53 (producing the protein p53), discovered more than 40 years ago.

Scientists certainly know a lot about p53. It starts a broad cascade of events inside the cell, involving the activation of hundreds of other genes, that will collectively try to control damage or, as a last resort, kill the cell for the benefit of the organism. However, the direct effects of p53 right after its activation remained poorly understood and this is of utmost importance, since abnormal inactivation of p53 is a known hallmark of cancer progression.

The researchers, led by Dr. Biola M. Javierre, developed an experimental setting able to detect the fast changes occurring as short as one hour after p53 activation and found it produced a heavy remodelling of the chromatin – the DNA inside the nucleus- consistent with the activation of its targets. The results were published at the high impact journal Nature Communications and involved colleagues from the Graupera and the Sardina labs, also at the Josep Carreras Leukaemia Research Institute, as well as researchers from the Barcelona Supercomputing Center, the University College London and the Florida State University.

Overall, the study identified 340 p53 target genes being directly activated through long-range interactions, involving the contact of specific activation regions in the genome – enhancers - with the genes themselves. That’s why chromatin had to be physically reorganized within the nucleus. Noteworthy, 70% of the genes had not been previously linked with p53.

The team also proved that the rewiring of the chromatin was dependent on the presence of the cohesin complex, a known group of proteins in charge of stabilizing DNA loops, precisely the ones allowing the long-range interactions between enhancers and genes, strengthening their findings.

Despite being a key player in cancer suppression, very few therapeutical strategies have been directed towards restoring p53 function in tumours where it is inactivated, either directly or through its secondary targets. Perhaps, its newly reported mechanism of early action and the identification of many more genes under its control can inspire new approaches hitting the clinic in the future.

The research has been funded with grants from the Spanish Ministry of Science and Innovation, the European Hematology Association and the Scientific Foundation of the Spanish Association Against Cancer. Some of the authors are also funded by the European Union’s Horizon 2020 research and innovation program and the La Caixa Banking Foundation.