image: Response surface for mixture design of experiment that optimizes the proportions between cocoa bean peel, peptone, and ammonium phosphate added to the peach palm waste under solid state fermentation by Trichoderma stromaticum AM7 (CCMB617P) at 30 °C for 6 days, for amylase synthesis (using the special cubic model). The copyright is retained by the authors.
Credit: Dr. Andréa Miura da Costa, Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil
Amylases are among the most important biotechnological and industrial enzymes that can be applied in various sectors, such as food, pharmaceuticals, textiles, chemicals, paper, and detergents.
The enzymes’ costs come from a range of factors including the quantity produced, the production process, the expense of its recovery, and the degree of purity at which it will be marketed, etc. The use of agro-industrial substrates and microorganisms brings the potential to low-cost enzyme production. Meanwhile, due to the ability to improve physical and chemical resistance to industrial environmental extremes, such as high temperature and pH, as well as carry out different catalytic reactions, microbial enzymes are favorite to meet the demands of the industry.
This innovative study led by Dr. Andréa Miura da Costa (Universidade Estadual de Santa Cruz) reported the amylase production by Trichoderma stromaticum AM7 using peach-palm waste as substrate.
A mixture simplex centroid design of the experiment was performed and the surface response methodology was applied to determine the mathematical model that describes the relation between the response variable and the independent factors (nitrogen source proportions). The team found that the design of the optimum proportion for the final mixture was 70% peptone, 24.3% ammonium phosphate, and 5.7% cocoa bean peel. After the addition of nitrogen sources as peptone and ammonium phosphate, the amylase production increased by 45% by T. stromaticum AM7.
The researchers also determined the pH and temperature optimum for amylase stability, as well as the effects of metal ions and chemical reagents on amylase activity. They found that the biochemical properties of the amylase showed its stability to acidic pH (4.0) and high temperatures up to 50–60 °C; In addition, the enzyme was little influenced by the presence of ions and denaturing agents, which gives the characteristic of the T. stromaticum AM7 amylase promising for applications mainly in the food sector.
In this innovative research, the fungus T. stromaticum AM7 was efficient in producing amylase using peach palm waste as low-cost substrate in solid state fermentation which simplifies and makes fermentation economically viable for this enzyme production. “The cost-effective enzyme extraction and stable amylase highlight its strong potential in industry applications, especially in food processes”, Dr. Andréa Miura da Costa said.
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About Mycology
Mycology —— An International Journal on Fungal Biology is a renowned international, peer-reviewed, open access journal that publishes all aspects of mycological research, sponsored by the Institute of Microbiology, Chinese Academy of Sciences and the Mycological Society of China. Since its inception in 2010, Mycology received strong support from mycologists around the world. By publishing cutting-edge research and facilitating collaborations, Mycology strives to advance the understanding and knowledge of mycology, while fostering innovative research and scientific discussions in the field.
Journal
Mycology: An International Journal on Fungal Biology
Article Title
Utilization of Peach-palm waste for cost-effective amylase production by Trichoderma stromaticum: Stability and industrial potential
Article Publication Date
12-Nov-2023