Unveiling the reproductive secrets of red-swamp crayfish

Procambarus clarkii is a crustacean species native to American freshwater bodies. Brought to Japan in the 1920s as a feed for frogs, this crustacean has slowly taken over the Japanese freshwaters by predation and propagation. In fact, in 2023, the red-swamp crayfish was designated as an invasive alien species by the Japanese Ministry of the Environment and of the Agriculture, Forestry and Fisheries.

One way to control the growth in population of red-swamp crayfish and the accompanying crisis is to capture the red-swamp crayfish in their reproductive form. To this end, however, it is essential to understand their reproductive characteristics, including their anatomical alterations. However, little is known about the reproductive biology of P. clarkii, not only in Japan, but also in their habitats elsewhere around the world. To address this research gap, researchers led by Professor Kazuyoshi Nakata of Okayama University, recently studied several reproduction aspects and morphological characteristics of the red-swamp crayfish. Mr. Quang-Tuong Luong, Ms. Rika Shiraishi, and Dr. Koki R. Katsuhara were also a part of this study, which was published in the Journal of Crustacean Biology on October 31, 2023.

To undertake this study, the researchers first collected the red-swamp crayfish over a span of one year, from November 2015 to November 2016, from a local pond in Okayama, western Japan. They collected and observed a total of 6,319 crayfishes, comprising 2,601 males and 2,777 females, with eight of unknown sex and 933 juveniles.

Red-swamp crayfish have four growth stages—eggs, hatchlings, juveniles, and adults. Adults alternate from being in a sexually active form (Form I) to a sexually inactive form (Form II). In the breeding season that peaks in autumn, it was observed that males in Form I had enlarged chelae, or the claw-shaped structures that crustaceans utilize for defense and capturing prey. By identifying this characteristic, this study is the first to highlight that hook length is an important indicator of sexual status in red-swamp crayfish. Hooks are the sharp horn-like extensions present on the third and fourth feet of the red-swamp crayfish. Most importantly, hooks are used by the males to secure the female in the mating position. It was visibly larger in Form I males than in Form II males. Confirming their observation, Prof. Nakata from the Faculty of Environmental, Life, Natural Science and Technology, Okayama University, Japan says, “Our results indicate that both chelae and hook length in males significantly differed among Form I and Form II, revealing chelae or hook length to be an important identifier for Form I or II males of P. clarkii.”

Moreover, the results also clarified that the invasive red-swamp crayfish P. clarkii shows a high reproductive ability and a fast growth rate compared to the Japanese native crayfish C. japonicus. It was observed that the female red-swamp crayfish could carry as many as 100 to 375 eggs per individual, whereas the Japanese native crayfish could carry only about 18 to 75 eggs. In red-swamp crayfish, even though the optimum temperature for egg hatching was found to be 25 °C, egg hatching could occur at temperatures as low as 7 °C. The cumulative degree for egg-to-juvenile maturation was found to be 1,222 °C.

Explaining these findings, Prof. Nakata says, “From this temperature value, it was possible to estimate the spawning date for juveniles. Moreover, the hatching dates and the time of juveniles leaving the females can also be calculated.” While the native Japanese crayfish takes approximately five years to reach reproductive age, the red-swamp crayfish invasive species reaches this age in the fastest case within just five months of hatching, which explains the fast growth rate of this highly invasive species.

Going ahead, these findings will contribute to promoting effective control of the invasive populations of P. clarkii and aid in the conservation of biodiversity in freshwater areas around the world.

About Okayama University, Japan

As one of the leading universities in Japan, Okayama University aims to create and establish a new paradigm for the sustainable development of the world. Okayama University offers a wide range of academic fields, which become the basis of the integrated graduate schools. This not only allows us to conduct the most advanced and up-to-date research, but also provides an enriching educational experience.

Website: https://www.okayama-u.ac.jp/index_e.html

About Professor Kazuyoshi Nakata from Okayama University, Japan

Dr. Kazuyoshi Nakata is a Professor at the Faculty of Environmental, Life, Natural Science and Technology at Okayama University, Japan. His areas of interest include conservation, ecology, and civil engineering, with a focus on endangered or invasive freshwater animals including fish, crustaceans and frogs etc. With over 70 publications, he has authored books such as ‘Shrimp, Crab, and Crayfish: Conservation and Biology of Freshwater Crustaceans.’ He has also been a contributor to the ‘Okayama Prefecture Red Data Book 2020’ that conducts surveys of animal populations and enlists endangered species. Apart from crustaceans, his research highlights conservation of an endangered bitterling fish Rhodeus atremius suigensis and ecological aspects of the Daruma frog, an endangered species from western Japan. Dr. Nakata was previously associated with Hokkaido University.