Fig. 2 (IMAGE)

Osaka University

Fig. 2

Caption

Genome editing using the Cas9 mutant, nickase. Nickase induces single-strand breaks (nicks) instead of DNA double-strand breaks. Nicks are typically repaired without causing mutations. Hence, even if nicks appear at sites different from nickase's original target, new gene mutations are seldom seen at these locations. This approach allows for precise gene correction with minimal off-target mutations.

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S. Nakada

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