Scheme of generating blastoids from TBLCs (IMAGE)
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To assess whether TBLCs possess the ability to form blastoids, 5, 10, 25, and 50 cells of mCMG-TBLCs were aggregated per microwell of the AggrewellTM 400 in the reported medium for generating EPS-blastoid (iBlastoid medium). After 5 days of culture, some of the microwells initiating with 5 and 10 cells turned out to be empty because of cell death. Nevertheless, most of the remaining cell clumps with different initiating cell numbers started to form a cavity. The cavities gradually grew bigger to form a blastocyst-like structure in another 2 days.
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