Scheme of generating blastoids from a single TBLC. (IMAGE)
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For high-throughput screening of the effects of gene manipulation in early embryogenesis, generating blastoids from a single TBLC is crucial. They cultured the single TBLC in a PlaB media supplemented with 50% irradiated mouse embryonic fibroblasts (iMEFs) conditional media in microwells for 4 days when the cell numbers were 16–22 and then changed the medium to iBlastoid medium. After another 7 days of culture, although around 34.8% of the microwells were blank, almost 30% of the surviving cell clumps formed blastocyst-like structures.
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