force fields of intact cells (IMAGE)

The University of Hong Kong

force fields of intact cells

Caption

Applying LPM to measure in-plane traction force fields of intact cells. (a) Schematic illustration showing the design of cell experiments: NDs are randomly embedded in an elastic substrate (PDMS) serving as fiducial markers; before (upper) and after (lower) cell lysis (3T3 cells treated with a detergent ), the orientation and position of NDs are measured via the LPM method. Red dashed arrow represents the projection direction of the NV center axis on the sample plane. (b) Typical bright-field (left panel) and scattering (middle panel) images of a cell fed with NDs. Fluorescence image (right panel) of a chosen ND with single NV center as indicated by the inserted g(2)(τ) measurement. White line (in the middle panel) outlines the profile of the cell. (c) A table shows the relative motions (before and after cell lysis) of a target ND with single NV center.

Credit

The University of Hong Kong

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