Preparation, characterization, lipid peroxidation, and MD simulations of Lp-IO. (IMAGE)

Science China Press

Preparation, characterization, lipid peroxidation, and MD simulations of Lp-IO.

Caption

(a) Schematic preparation process. (b) Cyro-TEM image of Lp-IO. (c) AFM (left) and MFM (right) images of Lp-IO. (d) X-band EPR spectra of •OH. (e&f) Lp, Lp-IO, and Lp+IO were separately treated in the presence or absence of H2O2. (e) The relative ratio of FL1 (green FL) to FL2 (red FL) from C11-BODIPY. (f) The peak area ratio of PC, LPC, and SM lipid hydroperoxide to 12:0 LPC (internal standard). (g&h) Equilibrated snapshots of H2O2 interacting with the pure lipid bilayer and the IO-PEG-doped lipid bilayer. (i) PMF profiles for a single H2O2 to penetrate the lipid bilayers. (j&k) Equilibrated snapshots of the interaction between •OH and lipid bilayers. (l) PMF profiles for a single •OH to penetrate lipid bilayers.

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