TIS window for two-photon microscopy. (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

TIS window for two-photon microscopy.

Caption

a1 A large-field and dual-channel two-photon microscopic image of bilateral hemispheric cortical blood vessels (in red, Texas red) and neurons (in green, EGFP) after the TIS window establishment. a2-a4 High magnification images of the corresponding color frames in (a1) near the sutura (viewed with a 20× objective). a5-a10 High magnification (zoom in) images of the white frames (white arrow-indicated) in (a2)-(a4), respectively. b Typical images of two-photon microscopy of neurons at various depths before and after the TIS window establishment, as well as after the removal of the skull. c The SBRs of the images at various imaging depths under different conditions. d Comparison between the TIS window and open-skull glass window in dendritic spine imaging. The excitation wavelength was 920 nm (80 MHz, 140 fs).

Credit

by Dongyu Li, Zhengwu Hu, Hequn Zhang, Qihang Yang, Liang Zhu, Yin Liu, Tingting Yu, Jingtan Zhu, Jiamin Wu, Jing He, Peng Fei, Wang Xi, Jun Qian and Dan Zhu

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License

CC BY

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