The comparison of T4 ligase-based method and SPAAC-based method. (IMAGE)

Science China Press

The comparison of T4 ligase-based method and SPAAC-based method.

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The previously reported CBApt construction technology faces several challenges, including the use of T4 ligase, and the need for 13 additional complementary nucleosides at the end of each aptamer. The newly developed strain-promoted alkyne-azide cycloaddition (SPAAC) strategy is a practical method to access CBApts because of its mild reaction conditions, ease of operation, and high compatibility with respect to aptamer conformations.

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