Fig. 2 (IMAGE)
Caption
Top, a 1455-nm infrared laser light was focused using an objective lens to apply heat to cells under a fluorescence microscope. The cells expressed RyR1 with mutations that are known to be related to malignant hyperthermia. Bottom, Ca2+ fluorescence imaging showed that intracellular Ca2+ levels increased within one second after heating began. The images are shown in a pseudo-color, with red indicating areas where the Ca2+ concentration was higher than the yellow or blue areas.
Credit
2022, Kotaro Oyama et al, Heat-hypersensitive mutants of ryanodine receptor type 1 revealed by microscopic heating, Proceedings of the National Academy of Sciences, U.S.A.
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License
CC BY