Dual-color OLID-SDOM imaging of lysosomes and actin in U2OS cells (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Dual-color OLID-SDOM imaging of lysosomes and actin in U2OS cells

Caption

a-b Conventional wide-field and OLID-SDOM image of Alexa Fluor 568 phalloidin labeled F-actin (red), and GFP-lamp1 labeled lysosome (green). Scale bar: 5 μm. c-e Expanded view of the dual-color wide-field image, SDOM image and OLID-SDOM image of F-actin (red) and lysosome (green) of the white dotted box in a-b. Scale bar: 1 μm. The direction of the carmine and yellow lines denotes the dipole orientation of F-actin and lysosome, respectively. f and g show the expanded view of wide-field and super-resolution images of transfected actin filaments of the white box in a-b, respectively. The inset shows the normalized intensity profiles taken from the regions indicated in f and g. Scale bar: 1 μm.

Credit

by Meiling Guan, Miaoyan Wang, Karl Zhanghao, Xu Zhang, Meiqi Li, Wenhui Liu, Jing Niu, Xusan Yang, Long Chen, Zhenli Jing, Micheal Q Zhang, Dayong Jin, Peng Xi, Juntao Gao

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License

CC BY

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