Principle and flowchart of OLID-SDOM (IMAGE)
Caption
a Rotary-linear-polarized illumination scheme of OLID. b Top: the sync signals of camera readout (blue line) and motor rotation pulse (red line). Middle: intensity curve of the laser after passing through a polarizer parallel to the X-axis of the system on the focal plane. Bottom: the emission intensity curve of a GFP molecule on the focal plane under polarization modulated excitation. There are four modulation cycles in one motor rotation cycle. c Alexa Flour 561 labeled F-actin in fixed mouse kidney sections, with wide-field image on the left, OLID image on the right. Scale bar: 5 μm. d The flowchart of OLID-SDOM. The data of four modulation cycles at each pixel were transformed by 1D Fourier transform, and then we extracted the modulated frequency component and generated a 1D single period data. After processing each pixel, 3D deconvolution of the obtained single period image, named as OLID image, was carried out to obtain the , and , and orientation mapping was extracted by FFT phase extraction.
Credit
by Meiling Guan, Miaoyan Wang, Karl Zhanghao, Xu Zhang, Meiqi Li, Wenhui Liu, Jing Niu, Xusan Yang, Long Chen, Zhenli Jing, Micheal Q Zhang, Dayong Jin, Peng Xi, Juntao Gao
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License
CC BY