High-speed Zebrafish blood flow imaging in 3D under extremely low illumination power (IMAGE)
Caption
(a) and (b) reconstructed zebrafish blood cell volumes by LFM and the DiLFM at z=-30 µm under 0.15 mW/mm2 (488nm) and 0.12 mW/mm2 (561nm) illumination, respectively. Red color shows the blood cells and green color shows blood vessel walls. (c) Zoom-in area marked by white dashed box in (a) and corresponding maximum intensity projection (MIP) along the y-axis by RL at different time stamps. (d) Zoom-in area marked by white dashed box in (b) and corresponding MIP along the y-axis by DiLFM at different time stamps. (e) Zoom-in area marked by white dashed box in (a) by LFM (left column) and in (b) by DiLFM (right column). (f) Time-lapse reconstructed intensity along the white line in (a) by LFM (top) and by DiLFM (bottom) at z=-30 µm. (g) and (h) Zoom-in area around the white line in (a) and (b) in 30ms time window by LFM (top) and DiLFM (bottom). The time window is indicated by the dashed box in (f). Arrows mark the blood cell at z=-30 µm at different time stamps. The white lines in (g) and (h) are the same as the lines in (a) and (b). Scale bars in (a) and (b) are 50 µm, in (c), (d), and (e) are 10 µm, in (g) and (h) are 5 µm.
Credit
by Yuanlong Zhang, Bo Xiong, Yi Zhang, Zhi Lu, Jiamin Wu and Qionghai Dai
Usage Restrictions
Credit must be given to the creator.
License
CC BY