Ciona egg before and after fertilization (VIDEO) Marine Biological Laboratory This video is under embargo. Please login to access this video. Caption Left: Ciona egg before and after fertilization (Movie of Total Internal Reflection Fluorescence Microscopy [TIRFM] images; F-actin probe is AF488-phalloidin). Right: Transient changes of F-actin alignments from the time before fertilization to the first cell division of Ciona egg. Positions of AF488-phalloidin particles bound to F-actin are shown as yellow dots, and the orientation of F-actin is shown as yellow bars. Frame intervals, 10s. Replay speed, 15 frames/s. Credit Hiro Ishii and Tomomi Tani Usage Restrictions Please use with credit. License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.