The Technique at a Glance (IMAGE)

Institute for Basic Science

The Technique at a Glance

Caption

Eggs from healthy donors and sperm from a man affected by familial hypertrophic cardiomyopathy are fertilized in vitro. (A) Previously, CRISPR/Cas9 (scissors) was injected into the egg after in vitro fertilization and could have acted after duplication of the DNA had already begun, running the risk of creating mosaic embryos, a mismatched situation where some cells are still mutated while other cells have been repaired. (B) The new technique injects CRISPR/Cas9 and the sperm at the same time, avoiding mosaicism problems.

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(Modified from Ma H. et al., Nature 2017)

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