Figure 2: Studying Autophagy with Fluorescence Resonance Energy Transfer (IMAGE)

Institute for Basic Science

Figure 2: Studying Autophagy with Fluorescence Resonance Energy Transfer

Caption

Cy3 emits fluorescence with a peak wavelength at 563 nanometers (black line), while Cy5 at 670 nanometers (red line). In the experiment, Cy3 is attached to CB[7] and Cy5 to AdA. During autophagy, the two dyes come closer and Cy3 transfers some energy to its nearby partner dye Cy5, following the FRET phenomenon.

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