Figure1 (IMAGE)
Caption
USP17 deubiquitinates to stabilize the c-Myc protein. A. 3HA-c-Myc was co-expressed with FLAG-USP17 (WT or the catalytically inactive mutant (C89S)) in COS7 cells. After 24 h, cell lysates were immunoblotted with the indicated antibodies. CS, C89S. B. PC-3 cells were transiently transfected with the indicated siRNAs. After 72 h, an immunoblot analysis was performed with the indicated antibodies. Ctrl, control. C. An empty vector or plasmid expressing USP17 WT or C89S was co-expressed with 3HA-c-Myc and a FLAG-Ub plasmid into COS7 cells. Cells were treated with 20 microM MG132. After 4 h, cell lysates were analyzed by a pulldown assay and immunoblotting with the indicated antibodies. CS, C89S.
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Nagoya City University
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