Immunostaining & Coimmunostaining (IMAGE)

Niigata University

Immunostaining & Coimmunostaining

Caption

Immunostaining: Top panel SH-SY5Y cells transfected with GBA, ATP13A2, and PINK1 siRNAs are stained for dsDNA (magenta), histone H2B (green) and Hsp60 (turquoise). White arrows indicate cytosolic dsDNA of mitochondrial origin. Triple siRNA: Knockdown of GBA, ATP13A2, and PINK1 expression with siRNAs. Coimmunostaining: Bottom panel In situ hybridization of mitochondrial DNA and coimmunostaining for histone H2B (green) and Hsp60 (turquoise) in SH-SY5Y cells transfected with GBA, ATP13A2, and PINK1 siRNAs. White arrows indicate cytosolic dsDNA of mitochondrial origin. Triple siRNA: Knockdown of GBA, ATP13A2, and PINK1 expression with siRNAs.

Credit

Matsui et al., Nat Commun. 2021

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