ASLM and 2-Photon Microscopy (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

ASLM and 2-Photon Microscopy

Caption

a, Genetically encoded multimeric nanoparticles inside two MV3 cells, as imaged by ASLM at 20 ms image integration time, and 3.57 volumes per second. b, YZ view of the perinuclear region. Yellow circles indicate detected vesicles and blue lines illustrate cumulative tracks. c, Schematic drawing of zebrafish embryo. d, Averaged (over 30 cycles) XZ cross section of zebrafish heart, acquired with a framerate of 45 Hz. e, Kymograph of beating heart, measured along line shown in d. Kymograph uses raw data and no averaging was applied. f, Volumetric imaging of a zebrafish heart at a volume rate of 7.4Hz, XY view with depth encoded in color. Scale bar, a, 10 microns; b, 1 microns; d,e 20 microns

Credit

by Tonmoy Chakraborty, Bingying Chen, Stephan Daetwyler, Bo-Jui Chang, Oliver Vanderpoorten, Etai Sapoznik, Clemens Kaminski, Tuomas P.J. Knowles, Kevin M. Dean, and Reto Fiolka

Usage Restrictions

None

License

Licensed content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.