Schematic Illustration (IMAGE)
Caption
a, A collimated laser beam is delivered into the setup by a beam-splitter (BS) and onto a galvanometric scanning mirror (GSM), which is imaged into the back focal plane of an air objective (OBJ1). Scanning the GSM rasters the focus in one dimension as shown by the double-headed arrow in the boxed front focal space of OBJ1. A step mirror reflects the light with different amounts of defocus back into the objective, which then travels through the lenses onto the GSM, where it is de-scanned by, which removes the lateral scan motion and only the axial component remains. The GSM is then again imaged onto the back focal plane of a water dipping objective (OBJ2). OBJ2 forms an aberration free image of the focus (as formed by OBJ1) in the sample space. b, Zoomed in view of the boxed region from a. Panel on the left shows the focus of the light at its nominal focus. Black arrows show returning marginal rays after reflection. Each step on the mirror results in a focus spot in the sample plane with a displaced axial position. c, Alternative configuration with a tilted mirror that allows continuous axial scanning. Here, the remote objective OBJ1 is slightly shifted off the optical axis to create a tilted focus that is incident normal to the mirror surface. Scanning this focus laterally results in a change of focus, as illustrated by the black arrows
Credit
by Tonmoy Chakraborty, Bingying Chen, Stephan Daetwyler, Bo-Jui Chang, Oliver Vanderpoorten, Etai Sapoznik, Clemens Kaminski, Tuomas P.J. Knowles, Kevin M. Dean, and Reto Fiolka
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