Ex vivo injection of Bmal1 shRNA plasmid DNA in the SCN slice of a Per1::luc Tg mouse using an NTW array for knockdown of Bmal1 expression (Fig3) (IMAGE)
Caption
(A) Three-dimensional images of the GFP signal in the Bmal1 shRNA plasmid DNA within the SCN slice after NTW-array injections. White arrows indicate SCN cells injected with Bmal1 shRNA using an NTW array. A white dashed circle indicates the whole area of the ventrolateral SCN, and a red dashed circle shows the core of the dorsomedial SCN. Most Bmal1 shRNA injected cells with GFP signal are identified in the white circle area mainly with pacemaker neurons. (B, C) The luciferase emission rhythms from the SCN slice of Per1::luc Tg mice injected with Bmal1 shRNA (B) or only a buffer (c) using an NTW array with a final concentration of 100 µM luciferin, and detrended.
Credit
COPYRIGHT (C) TOYOHASHI UNIVERSITY OF TECHNOLOGY. ALL RIGHTS RESERVED.
Usage Restrictions
None
License
Original content