CHAMP: Chip Hybridized Affinity Mapping Platform (IMAGE)

University of Texas at Austin

CHAMP: Chip Hybridized Affinity Mapping Platform

Caption

A stitched image of fluorescently-labeled DNAs on the surface of a Next-Generation Sequencing flowcell. Images were collected on a prism-TIRF microscope and used in the Chip Hybridized Affinity Mapping Platform (CHAMP), a newly described framework for high-throughput, quantitative analysis of protein-DNA interactions on synthetic and genomic DNA. With CHAMP, Jung et al. revealed the highly-specific sequence dependence for target binding and nuclease recruitment by Cascade, a Type 1 CRISPR-Cas system.

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Stephen Jones Jr./Univ. of Texas at Austin

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