Conceptualization and materialization of reaction flux derailing (RFD) approach (IMAGE)
Caption
The RFD strategy is conceptualized from the hypothesis that the metabolic intermediates or precursors may derail the reaction flux if reacting with chemically similar substrate analogs. The RFD approach is designed and validated by the individual addition of 5-methoxyindole and 5-chloro-indole-3-carbinol (5-Cl-I3C) into the I3C oligomerization mixture. The labelled compounds formed therein signify that LTr1, DIM, 3MI, and formaldehyde are involved in the I3C-to-LTr1 reaction flux. Using the RFD methodology, Tan’s group clarifies how and why LTr1, DIM, and I3C are formed and/or converted in different gastrointestinal compartments in mice. I3C, indole-3-carbinol; DIM, 3,3'-diindolylmethane; LTr1, 2-(indol-3-ylmethyl)-3,3'-diindolylmethane; 3MI, 3-methyleneindolium.
Credit
©Science China Press
Usage Restrictions
Use with credit.
License
Original content