Integrated Blood-Barcode Chip -- Diagram (IMAGE)
Caption
This figure represents an artist's drawing (more or less to scale) of the channel through which the whole blood is flowed, and three of the plasma-skimming channels. The cells shown are mostly red blood cells, with a few white blood cells and platelets. Barcodes are shown in each of the three plasma-skimming channels, and the expanded view of a barcode illustrates some of the salient aspects of the assay. The different colors of the stripes correspond to different stripes of ssDNA. Antibodies are assembled onto specific stripes via DNA hybridization, just prior to execution of the assay. As the plasma flows through the skimming channels, proteins bind to their cognate antibodies. After plasma flow is completed, the assay is developed by introducing fluorescently labeled, secondary antibodies. The green stripe in each of the barcodes serves as an alignment marker, and the individual proteins are detected by their spatial location relative to this stripe. Each of the plasma skimming channels contains between 30 and 50 complete barcodes, and so protein levels are assessed by averaging over many barcodes readouts.
Credit
Drawing courtesy J. Heath, R. Fan, and H. Amad
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