Figure 2 | Intracellular imaging of subcellular structures (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Figure 2 | Intracellular imaging of subcellular structures

Caption

(a) Fluorescence images of the microfilaments (F-actin filaments) of a living adipose cell. The weak fluorescent signals (a1) were efficiently enhanced by the lipid droplet (diameter: 7.7 μm) (a2). (b) Fluorescence images of the microfilaments in a fixed adipose cell with an 18.6-μm lipid droplet. (c) Fluorescence and bright-field images of the lysosomes in a living adipose cell with an 11.3-μm lipid droplet. (d) Fluorescence and bright-field images of the adenoviruses in a living adipose cell with an 8.1-μm lipid droplet, the blue lines represent the movement trajectory of the adenoviruses in 10 min. The fluorescence images were focused on the surfaces of the cells (b1) and the virtual image planes (b2, c1 and d1) formed by the lipid droplets. The bright-field image was focused on the virtual image plane formed by the lipid droplet (c2 and d2).

Credit

by Xixi Chen, Tianli Wu, Zhiyong Gong, Jinghui Guo, Xiaoshuai Liu, Yao Zhang, Yuchao Li, Pietro Ferraro, and Baojun Li

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License

CC BY

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