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Caption

The axon of a neuron has rings of actin spaced every 190 nm by spectrins (A). Ultrasonic unroofing (B) makes the axon accessible using platinum-replica electron microscopy (PREM). This allows to visualize rings formed of two long actin filaments (magenta and arrow points, C). Using stochastic optical reconstruction microscopy (STORM) and electron microscopy (D) we can identify the actin rings (orange and arrow points) within the spectrin network.

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Christophe Leterrier & Stéphane Vassilopoulos/CC

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