[Figure 3] Images of the Mouse Brain Obtained Using Reflection-Matrix Microscopy (IMAGE)

Institute for Basic Science

[Figure 3] Images of the Mouse Brain Obtained Using Reflection-Matrix Microscopy

Caption

[Figure 3-1] Label-free reflectance imaging of myelinated axons in a mouse brain through the intact skull (a) Skull and brain sample to be imaged. (b) A reflectance image measured by the conventional optical coherence microscopy. The thickness of the skull was about 100?μm. (c) Aberration-free high-resolution image obtained by the reflection matrix microscopy. (d) Phase maps of wavefront aberrations for small sub-regions of the image found by a new aberration correction algorithm. [Figure 3-2] Demonstration of aberration correction in two-photon fluorescence imaging through an intact mouse skull. (a) and (b) Two-photon fluorescence images of neuronal dendrites obtained at two different depths. (c) and (d) Images after physically correcting aberrations by SLM. The thickness of the intact skull was about 85 μm.

Credit

IBS

Usage Restrictions

Please mention credit

License

Licensed content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.