Figure 2. Visualizing RNA Polymerase II and Histone Modifications in Living Embryos (IMAGE)

Tokyo Institute of Technology

Figure 2. Visualizing RNA Polymerase II and Histone Modifications in Living Embryos

Caption

(A) Scheme of experiments. Fluorescently labeled Fabs prepared from modification-specific antibodies are injected into 1-cell-stage zebrafish embryos, which are then mounted for a lightsheet (SiMView) or a confocal (FV1000) microscope. (B) Representative images taken with a SiMView microscope. Fabs specific to active, phosphorylated RNA polymerase II (RNAP2 Ser2ph) and histone H3 acetylated at the 9th lysine (H3K9ac) were simultaneously injected and imaged. RNAP2 Ser2ph Fabs were clearly concentrated in nuclei around the 1k-cell stage, whereas H3K9ac Fabs were enriched in nuclei from the 8-cell stage. Scale bar: 100 μm.

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Tokyo Tech, Development

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