CaV2.1 Can Be Selectively Ablated and Functionally Rescued at the Calyx of Held Terminals (IMAGE)

Max Planck Florida Institute for Neuroscience

CaV2.1 Can Be Selectively Ablated and Functionally Rescued at the Calyx of Held Terminals

Caption

A, Cartoon depicting CaV2.1 α1 subunit distal C-terminal interaction partners. B, left: Schematic view of stereotactic surgery to inject/coinject HdAd vectors expressing Cre + eGFP and CaV2.1 constructs + mCherry into the aVCN at age P1. Right: top: Experimental timeline from virus injection at P1 to electrophysiological recordings at P9-P11 prior to the onset of hearing (P12). Middle and bottom: schematic view of the viral constructs used, expressing either Cre + eGFP or CaV2.1 constructs + mCherry, respectively, driven by individual promotors. C, Calyx of Held terminals transduced with Cre + eGFP (top) and CaV2.1 + mCherry (middle). eGFP and mCherry signals overlap with those of a calyx of Held loaded with Lucifer Yellow via a patch pipette (bottom).

Credit

Max Planck Florida Institute for Neuroscience

Usage Restrictions

None

License

Licensed content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.