Figure 2 (IMAGE)

Institute for Basic Science

Figure 2

Caption

PAM compatibilities for SpCas9 variants. (a) Darker colors indicates higher frequency of DNA cleavage. (b) Among these four variants (SpCas9, VRQR, xCas9 and SpCas9-NG), SpCas9-NG has been the traditional choice for all PAM sequences that have a guanine (G) as the second nucleotide. However, these results shows that for PAM sequences AGAG and GGCG, for example, the Cas9 variant VRQR (in blue) would be preferable.

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