Comparison between the Two Gene Scissors: the Third-Generation CRISPR-Cas9 Technique and the Base Ed (IMAGE)

Institute for Basic Science

Comparison between the Two Gene Scissors: the Third-Generation CRISPR-Cas9 Technique and the Base Ed

Caption

In the original CRISPR-Cas9 technique (top), the guide RNA (green) binds to the target DNA and the cleavage enzyme Cas9 (scissors) cuts out a small DNA sequence (red). A modified version of Cas9 called nCas9 (bottom) is different as it cuts off only one strand of DNA and the cytidine deaminase (pink) transforms a single cytosine (C) into uracil (U). Uracil (U) is then converted to thymine (T) by DNA replication.

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