Schematic Representation of Procedures for The Generation of Gene Disruption Lines (IMAGE)

Kumamoto University

Schematic Representation of Procedures for The Generation of Gene Disruption Lines

Caption

These are the: 1. Construction of gene targeting vectors 2. Plant transformation 3. First generation plant selection 4. Gene disruption efficiency evaluation 5. Onset of genome editing events 6. Selection of plants that lost gene disruption tools (preventing unpredictable/extra genome editing) 7. Selection of plants containing target gene mutation 8. Establish mutant line/publish collection

Credit

Assistant Professor Takashi Ishida

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