Sperm Stored in Lifor (VIDEO)

Kumamoto University

Caption

Cauda epididymides were taken from sacrificed male mice and stored in Lifor (Lifeblood Medical Inc., NJ, USA) perfusion medium at 4C for 7 days. After storage, sperm were isolated and incubated in BSA-free Toyoda Yokoyama Hoshi medium containing 0.75 mM MBCD and 1.0 mg/mL polyvinyl alcohol for 60 min.

Credit

Professor Naomi Nakagata

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