2. DHX36 deficiency causes abundant G-quadruplex accumulation on rRNA in mouse GV oocytes. (IMAGE)
Caption
(a) Distribution of PQS (G-score ≥ 30) within mouse 47S pre-rRNA (Genebank: BK000964.3). (b) FISH of pre-18S and pre-28S in GO and FGO. Triangles indicate the nucleolus or NLB in the oocytes. Scale bar, 20 μm. (c) Immunofluorescence staining of DHX36 in mouse GO and FGO. Triangles indicate the nucleolus or NLB of the oocytes. Scale bar, 20 μm. (d) Fluorescence imaging of BG4 in the nucleus of WT and CKO GO. Triangles indicate the nucleoli in the oocytes. Scale bar, 10 μm. (e) Biotin-labeled PQS (ITS1-Q1) and mutant sequence (ITS1-Q1-Mut) used for streptavidin pull-down assay. Western blot analysis of DHX36 enriched by streptavidin-pull down assay. (f) Molecular structure and end-stacking G4-binding model of CYTO. (g) Fluorescence imaging of CYTO in GO and FGO. Triangles indicate the nucleolus or NLB. Scale bar, 20 μm. (h) Fluorescence of CYTO signal in HeLa cells treated with or without CX-5461 (1 μΜ) for 24 h. Scale bar, 20 μm. (i) Fluorescence of CYTO signal in WT and CKO FGO. Triangles indicate the NLBs of the oocytes. Scale bar, 20 μm.
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