Loss of S1pr3 inhibited macrophage M2 polarization. (IMAGE)
Caption
(A) Representative images for co-immunostaining of CD115 and Arg1 in lung sections of S1pr3-C and S1pr3-CKO mice after BLM challenge, Scale bar = 50 μm. (B, C) Quantitative reverse transcription-PCR and Western blot analysis of lung Arg1 in S1pr3-C and S1pr3-CKO mice. (D) Western blot results for S1pr3, iNOS, and Arg1 expression in BMDMs after IL-4 induction. (E) Quantitative reverse transcription-PCR analysis of Arg1, Mrc1, Chi3l3, Fizz 1, and iNOS expression in BMDMs after IL-4 induction. (F) Flow cytometry analysis of the percentage of M1 macrophages (CD11b+F4/80+CD11c+CD206− cells) and M2 macrophages (CD11b+F4/80+CD11c−CD206+ cells) in BMDMs following IL-4 stimulation. (G) Time-course Western blot analysis of IL-4-induced PI3K/Akt-Stat 3 signaling activity in BMDMs. BMDMs, bone marrow-derived macrophages; Arg1, arginase 1; Mrc1, mannose receptor 1; Chi3l3, chitinase 3-like 3; Fizz1, found in inflammatory zone 1; ns, not significant. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Genes & Diseases
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