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Higher Education Press

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AOF-containing serum ameliorates lipid deposition in HK-2 cells under high glucose conditions by promoting PPARα. PPARα mRNA (A) and protein (B) expression levels were detected by RT-qPCR and Western blot, respectively after administration of 100 pmol si-PPARα synthesized by IBSBIO. Red oil O-stained positive area (magnification ×10; scale bar: 100 μm) (C); TC (D) and TG (E) contents; PPARα mRNA and protein expression (F-G) as well as its fluorescence intensity (magnification ×10; scale bar: 100 μm) (H); ACOX1 mRNA expression (I); CPT1A mRNA expression (J). All data are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.001.

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Zi-Jie Yan, Lin Zhang, Xin-Yao Han, Yu Kang, Shu-Man Liu, Tian-Peng Ma, Man Xiao, Yi-Qiang Xie

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