SPP1 is a novel macrophage marker in CRC. (IMAGE)
Caption
(A) Heatmap of the proportion of M0, M1, and M2 macrophages (CIBERSORT), immune score (ESTIMATE), expression of markers (CD45, CD68, CD86, iNOS, CD163, CD206, and SPP1), CD68/CD45, and SPP1/CD68 in 10 bulk RNA-seq datasets (NC vs. CRC). The color gradient of each tile signifies changes in the marker expression level, where red represents an increase in CRC, blue represents a decrease, and the depth of the color reflects the statistical significance. Additional information provided includes the analysis method (right), dataset source (top), and the sample sizes of NC and CRC for each dataset (bottom). (B) Quantitative real-time PCR analysis of SPP1 mRNA expression between 50 paired NC and CRC samples. Wilcoxon test; ∗∗∗P < 0.001. (C) Violin plots of the expression of SPP1 in CD45− cells, other immune cells, other myeloid cells, and SPP1+ macrophages (GSE178341). (D) UMAP plot of the distribution of CD45− cells, other immune cells, other myeloid cells, and SPP1+ macrophages (left). UMAP of iterative subsets of cells from the global level to immune cells to myeloid cells shows the enrichment of SPP1 expression levels in different cells (right). (E) Images of immunohistochemical staining showing SPP1 protein expression in NC, CRC, NL, and HCC samples. Data are from The Human Protein Atlas. Scale bar, 200 μm. (F) Significantly enriched GO BP (Gene Ontology Biological Process) terms in genes coexpressed with SPP1 in the TCGA-CRC cohort. SPP1, secreted phosphoprotein 1; CRC, colorectal cancer; NC, normal colorectum (adjacent colorectum); NL, normal liver (adjacent); HCC, hepatocellular carcinoma; iNOS, inducible nitric oxide synthase; FDR, false discovery rate.
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Genes & Diseases
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