Comparison of the EFS-IL2RG.wt and EFS-IL2RG.co vectors in human ED7R cells and the bone marrow stem cells. (IMAGE)

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Comparison of the EFS-IL2RG.wt and EFS-IL2RG.co vectors in human ED7R cells and the bone marrow stem cells.

Caption

(A) Scheme of the self-inactivating (SIN) lentiviral vector containing the deleted long terminal repeats (LTRs) (Δ), the short EF1α promoter (EFS), the codon-optimized or wild type version of the IL2RG (IL2RG.co/IL2RG.wt), the HIV-1 Rev Responsive Element (RRE), and a mutated Woodchuck Post-transcriptional Response Element (WPRE). (B) Flow cytometry of CD132 expression, IL2RG-mRNA expression, and quantitative PCR analysis of VCN in EFS-IL2RG.co and EFS-IL2RG.wt vector-transduced ED7R cells. IL2RG-mRNA expression is expressed as fold change with respect to endogenous IL2RG in Jurkat cells. (C) CD132 expression and VCN in EFS-IL2RG.co and EFS-IL2RG.wt vector-transduced human bone marrow-derived CD34+ cells. VCN, vector copy number; MFI, mean fluorescence intensity; MOI, multiplicity of infection.

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Genes & Diseases

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