Distribution characteristic of proliferating hepatocytes and hemodynamic changes in PVL-induced liver regeneration and expression of Piezo1 in various liver cells. (IMAGE)
Caption
(A) Schematic diagram of the rat PVL model. (B) Changes in the ratio of liver weight to body weight in each lobe after 70% PVL in rats (the changes of each liver lobe were compared with 0 h). (C) Immunofluorescence staining analysis of Ki67 (in PV-CV) in paraffin tissues from the right liver lobe at 0, 24, 48, and 72 h after 70% PVL. Scale bar: 100 μm. (D) Ki67-positive hepatocyte changes during liver regeneration in the PV zone, Ki67-positive hepatocytes were counted in three randomly chosen PV fields, and the average count of these three fields was set as the Ki67-positive cell number of one rat (the statistical analysis of each time point was obtained by comparing with the results of 0 h). (E, F) Ultrasound localization microscopy assessment of hepatic microvascular blood flow changes between 0 h and 0.5 h after PVL. (G, H) The expression level of Piezo1 (from single-cell RNA sequencing) in different cell subsets in the human liver (https://www.proteinatlas.org/, https://livercellatlas.org) and mouse LSECs (GEO data: GSE102419, GSE119547, and GSE181482). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; two-tailed Student's t-tests. PVL, portal vein ligation; LL, left lobe; ML, middle lobe; RL, right lobe; CL, caudal lobe; POT, post-operative time; PV, portal vein; CV, central vein; LSEC, liver sinusoidal endothelial cell.
Credit
Genes & Diseases
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND