miRNA sequencing analysis of bone mesenchymal stem cell-derived apoptotic bodies (BMSC-ABs)-loaded polycaprolactone (PCL) scaffolds driving the molecular reprogramming of macrophages (Mϕs) to M2-Mϕs. (IMAGE)
Caption
(A) The top 50 known miRNAs detected in BMSC-ABs. (B) miRNA-mRNA regulatory network. (C) The binding of miR-21a-5p to the target gene CCL-1 in 293T cells validated by dual luciferase assay. (D) Colocalization of miR-21a-5p with Mϕs. (E) Fluorescence intensity analysis. n = 3; ∗P < 0.05. (F) Western blot analysis of the effect of the miR-21a-5p inhibitor on the reprogramming of BMSC-ABs to Mϕs. (G) Relative grayscale values of the western blot analysis of miR-21a-5p inhibitor's effect on the reprogramming of BMSC-ABs that drive Mϕs to M2-Mϕs. n = 3; ∗∗∗P < 0.001. (H) Quantitative PCR analysis of miR-21a-5p inhibitor's effect on the reprogramming of BMSC-AB-driven Mϕs to M2-Mϕs. n = 3; ∗∗∗P < 0.001.
Credit
Genes & Diseases
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