Melatonin inhibits Ca2+ overload induced by ER stress upon Slc26a2 deficiency. (IMAGE)
Caption
(A) Representative transmission electron microscopy images of ER. (B) Statistical analysis of endoplasmic reticulum diameter. (C, D) Flow cytometry analysis of intracellular calcium concentration. (E, F) Co-staining of Fluo-3 AM (green), a fluorescent Ca2+ indicator, with ER tracker (red) and statistical analysis. Scale bar: 100 μm. (G–I) Immunostaining of ER luminal calcium release channel proteins, IP3R and RYR2, and statistical analysis. Scale bar: 100 μm. Statistical significance was assessed through One-way ANOVA followed by Tukey's multiple comparisons test. The results are presented as mean ± standard deviation, with ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 indicating statistical significance, while “ns” denotes no statistical significance. ER, endoplasmic reticulum; SLC26A2, Solute carrier family 26 member 2; CTR, cre-negative control; IP3R, inositol 1,4,5-trisphosphate receptor; RYR2, ryanodine receptor 2.
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Genes & Diseases
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