Ce6-GFFY suppresses the proliferation of colorectal cancer cells (IMAGE)
Caption
CT26 and HCT116 cells were treated with indicated agents at 37 °C for 1 h and then treated with or without 660 nm laser irradiation for 1 min at a power of 0.02 W/cm2. The data are representative of three independent experiments. (A) Cells were incubated at 37 °C for 24 h after being treated with laser irradiation and the indicated dose of GFFY peptide, Ce6, and Ce6-GFFY, and then cell proliferation was determined using CCK-8 assays. The IC50 of Ce6-GFFY under laser irradiation was analyzed. IC50, 50 % inhibitory concentration. (B) Cells (CT26, 10 μM; HCT116, 5 μM) were stained with annexin V and propidium iodine dye, and then the ratio of dead cells was analyzed using flow cytometry. (C) The ratio of necrotic and apoptotic cells in the combined treatment of Ce6-GFFY and laser irradiation were analyzed. (D–F) After treated with the indicated agents (CT26, 10 μM; HCT116, 5 μM), cells were incubated at 37 °C for 8 h and stained with a CRT antibody, then the expression of CRT was analyzed using flow cytometry (D) and confocal laser scanning microscopy (E), and the CRT fluorescence was analyzed (F). CRT, calreticulin; green, CRT; blue, DAPI; MFI, mean fluorescence intensity. Scale bar, 40 μm “L” in “CT26+L”, “HCT116+L”, “PBS + L”, “GFFY + L”, “Ce6+L”, “Ce6-GFFY + L”: laser irradiation. Statistical analyses were performed using one-way ANOVA; bars, standard deviation; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.
Credit
Wei Qiao, Shuxin Li, Linna Luo, Meiling Chen, Xiaobin Zheng, Jiacong Ye, Zhaohui Liang, Qiaoli Wang, Ting Hu, Ling Zhou, Jing Wang, Xiaosong Ge, Guokai Feng, Fang Hu, Rongbin Liu, Jianjun Li and Jie Yang
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