Figure 1. Mutation of GhCKI promoter by CRISPR/Cas9 and CRISPR/Cpf1. (IMAGE)
Caption
Figure 1:(A). Overexpressing plants of GhCKI showed obvious male sterility. (B) Substantially decreased the expression of GhCKI caused completely sterile in upland cotton. GhUBQ7 (Ghir_A11G011460) was used as control. (C). The distribution of sgRNA target sites cover around 2 kb of the promoter of GhCKI. (D). The accessible chromatin regions of GhCKI promoter in upland cotton anther at the tetrad stage under NT and HT at the single-cell level. The violin plots on the right represent the expression levels of GhCKI in each cell cluster. The single-cell level expression and chromatin accessibility of GhCKI were retrieved from the published integrated multi-omics atlas (RNA+ATAC) of upland cotton anthers. (E). Eight promoter-edited alleles of GhCKI were obtained, respectively. The deletion (−) base pairs are indicated by numbers. EP, epidermis; EN, endothecium; ML, middle layer; T, tapetum; MAM, microspore after meiotic; V, vascular region; C, connective; MC, meiotic cell; NT, normal temperature; HT, high temperature.
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