Figure 2. GhCKI promoter-edited alleles improve anther thermotolerance. (IMAGE)
Caption
Figure 2: (A). The normalized relative expression of GhCKI in upland cotton anthers at tetrad stage of promoter editing mutants and wild type controls under NT and HT were measured by RT–qPCR (three biological and three technical replicates). GhUBQ7 (Ghir_A11G011460) was used as the internal reference. Different letters indicate significant differences, as determined by ANOVA followed by Tukey’s multiple comparison test (P <0.05). (B). The image above represents the main editing status of the GhCKI promoter in each mutant. (C). Examination of male fertility of GhCKI promoter editing mutants under NT and HT. Scale bars above = 1 cm. Scale bars below= 200 µm. The red pollen grains represent fertile pollen grains and the white pollen grains represent sterile pollen grains. (D). Statistics on pollen viability rate and anther dehiscence rate of GhCKI promoter editing mutants under NT and HT. (E). Quantification of pollen activity using TTF values. The error bars represent standard error of the mean. Statistical analyses were performed using a Student’s t test: *P <0.05; **P <0.01. NT, normal temperature; HT, high temperature.
Credit
©Science China Press
Usage Restrictions
Use with credit.
License
Original content