Figure 2 (IMAGE)
Caption
Structural details at the inner surface of an opened MT. (a) AFM image of the inner surface organizations of αβ-tubulin heterodimers in the MT wall, acquired in PEM-G buffer. The presence of different molecular heights and orientations of tubulin protofilaments can be seen, marked by red and blue arrows. The white arrow indicates the direction of protofilament orientation. (b) AFM image of the inner surface of the MT wall shown in panel a, acquired at different scan angles, showing the submolecular structural details. (c) Enlarged AFM image of the framed region in (b), allowing the determination of the arrangement of αβ-tubulin dimers within the topographically lower PF, providing a high-resolution image of the tubulin subunits (magenta and green circles). (d) The height profile taken across the PFs on the inner surface (dashed black line in panel (b)).
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© 2024, American Chemical Society
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