Construction of cell model for knockdown of PFDN6. (IMAGE)
Caption
(A) The mRNA expression of PFDN6 in CRC cell lines was evaluated by qRT-PCR. (B) The knockdown efficiencies of shPFDN6-1, shPFDN6-2 and shPFDN6-3 were detected by qRT-PCR. (C, D) The mRNA and protein expression of PFDN6 in CRC cell lines (HCT-116 and RKO cell lines) was evaluated by qRT-PCR (C) and western blot (D), the relative expression was quantified by normalising to GAPDH. (E) Immunofluorescence detection of PFDN6 expression in CRC cell lines. GFP: PFDN 6, magnification: 400. The data are expressed as mean±SEM; *p<0.05, **p<0.01, ***p<0.001 vs shCtrl. CRC, colorectal cancer; GFP, green fluorescent protein; qRT-PCR, quantitative real-time PCR.
Credit
By Fenghua Xu, LingYang Kong, Xiao Sun, et al.
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