Stepwise model of reverse transcription by the prime editor (IMAGE)
Caption
Structure-based stepwise model of the prime editor. The pegRNA base-pairs with the nicked strand of target DNA to form the RNA–DNA heteroduplex on the surface of the RuvC nuclease domain (RNA–DNA formation). The reverse transcriptase recognizes the RNA–DNA heteroduplex and initiates reverse transcription (Initiation). The reverse transcriptase consistently engages in reverse transcription of the template sequence around the initiation site, and the elongated RNA–DNA heteroduplex accumulates along the longitudinal surface of SpCas9, accompanied by the rearrangement of the target DNA duplex (Elongation). The reverse transcriptase performs excessive reverse transcription beyond the template and terminates reverse transcription due to collision with Cas9, leading to undesired insertions (Termination).
Credit
Shuto et al 2024
Usage Restrictions
Credit must be given to the creator.
License
CC BY