Results of in silico assay. (A) Alignment of multiple MPR2 protein sequences across species. (IMAGE)
Caption
The ABCC2 c.1177C>T resulted in an R393W substitution in MRP2 protein in the conserved amino acid region in different species. *Represents the position of the R393 is indicated by the red box. (B) Prediction of amino acid values. Arrow and blue dot indicate the position of R393. (C) Localisation of R393 and L1211 in MRP2 by SWISS_MODEL. (D) In wild-type MRP2 protein, R393 forms hydrogen bonds with G389, M397, Q429 and N1193. In mutant MRP2 protein, the hydrogen bond between W393 and Q429, and N1193 were broken, and a new hydrogen bond was formed between W393 and M424. (E) In wild-type MRP2 protein, L1211 forms hydrogen bonds with L1207 and G1215. In mutant MRP2 protein, the hydrogen bond between P1211 and L1207 were broken.
Credit
By Sun R, Chen Y, Zhu M, et al.
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