Figure 2| T-SRS-based live-cell chemical imaging. (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Figure 2| T-SRS-based live-cell chemical imaging.

Caption

Label-free imaging of (a) proteins and (b) lipids of live cells. These two channels are unmixed from the overall hyperspectral data with the standard linear decomposition method. (c) Typical T-SRS spectra of proteins (region 1 in (a), red curve) and lipids (region 2 in (b), Green curve). (d) Typical T-SRS imaging of normal Hela cell and (e) 50-µM arachidonic acid (AA) cultured Hela cell in the [1570 cm-1, 1700 cm-1] range. (f) shows the spectra of the marked regions in (d) and (e), respectively. The red dashed curve is the C=C stretching mode Raman spectrum of pure AA. (g) Protein channel reference and (h) The C-D stretching band of d31- palmitic acid labeled cells. (i) T-SRS spectrum of the marked structure in (h). Scale bar: 10 μm for (a), (b), (d) and (e); 5 μm for (g) and (h).

Credit

by Qiaozhi Yu, Zhengjian Yao, Jiaqi Zhou, Wenhao Yu, Chenjie Zhuang, Yafeng Qi & Hanqing Xiong

Usage Restrictions

Credit must be given to the creator.

License

CC BY

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.